Comparison of thinprep needle rinse samples versus direct smear Essay

Comparison of thinprep needle rinse samples versus direct smear techniques for fine neddle aspiration biopsies of head and neck lesions - Essay Example As a result, the pathologists and clinicians are to depend on the conveniences of a particular method that is most pertinent to the circumstance. A series of researches was led on the comparative diagnostic accuracy of the two techniques of FNA and their popularity among the clinicians and the pathologists (Frost et al, 1998). An analysis of the studies that have been led on the evaluation and the development of the techniques of Fine Needle Aspiration show an encouraging support for the ThinPrep technique of Fine Needle Aspiration Biopsies and trace the significant attempts in avoiding the pitfalls in the technique. In general, researchers are of the opinion that the accuracy of FNA at any site in the head and neck depends on both the skill and knowledge of the sites of the individual who will perform the FNA biopsies. As Layfield (2007) says, ‘Studies have shown that FNA is most accurate when the individual interpreting the FNA is the same individual who obtained the aspirate material’ (p. 798). In order to evaluate the performance of the FNA at the sites in the head and neck studies have had to revolve around the following questions: As far as the procedure of biopsy involves the obtainment of tissue, cell, or other specimen for diagnostic purposes, Fine Needle Aspiration has always played a crucial role as an initial technique of collecting them from a number of sites of human body. Indeed FNA is applied at a number of sites within the head and neck. The pressing appeal of the technique lies in several factors such rapidity, inexpensiveness, high convenience, low morbidity and necessarily in a relatively high diagnostic accuracy (Lester 2007). The amenability that areas in the head and neck show to this technique is mainly due to its capability to overcome the structural obstructions and barriers of the sites such as

Occupational hygiene survey Essay Example | Topics and Well Written Essays - 1500 words

Occupational hygiene survey - Essay Example The weight of dust sample was used to determine the exposure level of dust. Besides, the samples were chemically analyzed to determine the level of resin acids. In addition to that the details of the subject, environmental factor like temperature and relative humidity was recorded Air samples were collected too for analysis of microbiological content. Sampling was done several areas. Samples were collected simultaneously for a sampling period of 5 minutes. A sample was collected outside the gate of the factory to be a representative of ambient condition. Samples were then sent back to the laboratory for analysis. A seven-hole inhalable dust sampler (JS holdings, UK) was used to collect dust sample. The sampler was with 0.45m pore sizes, 25mm diameter Teflon Filters (Costar). This device was used to collect sample inhalable, as if inhalation through one's nose. The resemblance of the mechanism allowed study on health hazards by the dust inhaled. Teflon filter were used in order to collect particles for resin acids analysis. In the study, air was drawn through the sampler at the speed of 2 litre/minute. Four samples were taken for each location respectively. The weight of dust was determined by subtracting the pre-weight of the filter from the post-weight. All the weight measurement was conducted on the micto-balance (0.001mg sensitivity). Samples were equilibrated to the same temperature and relative humidity. All readings were taken at temperature 20+0.5 C and 50% relative humidity. Resin Acid Analysis There are two categories of resin acids: abietic and pimaric acid. The amount of acids exist in the dust depends on the species, maturity and storage condition. Method used to analyze resin acid was developed by Victor Leong and Mary Ellen Cassinelli (Pengelly, 1994). The quantity of abietic and pimaric acid was determine by a gas chromatography methodology. From the previous analysis, the Teflon filters were shifted into 100mm glass tubes with phenolic caps. 3ml of dichloromethane was used to extract the filters under ultrasonic bath for 30minutes. The extract was filtered with 24mm diameter 0.45 m pore nylon syringe filters. It was then washed with 0.5 ml of dichlorometh